Clifford Stephan

Clifford Stephan

Research Associate Professor and Director

Center for Translational Cancer Research
2121 W. Holcombe Blvd
Houston, TX   77030

Phone: 713-677-7456
Fax: 713-677-7779
cstephan@ibt.tamhsc.edu

Education and Training

Vanderbilt University
Ph.D., Pharmacology
B.A., Chemistry & Molecular Biology

Postdoctoral Training
Brigham and Women’s Hospital, Harvard Medical School

Research Interests

  • Drug Discovery
  • High Throughput Drug Screening
  • Combinatorial Drug Screening

Peer Reviewed Publications

Vijay GV, Zhao N, Den Hollander P, Toneff MJ, Joseph R, Pietila M, Taube JH, Sarkar TR, Ramirez-Pena E, Werden SJ, Shariati M, Gao R, Sobieski M, Stephan CC, Sphyris N, Miura N, Davies P, Chang JT, Soundararajan R, Rosen JM, Mani SA. GSK3β regulates epithelial-mesenchymal transition and cancer stem cell properties in triple-negative breast cancer. Breast Cancer Res. 2019 Mar 7;21(1):37. doi: 10.1186/s13058-019-1125-0. PubMed PMID: 30845991.

Huang L, Brunell D, Stephan C, Mancuso J, He B, Thompson TC, Zinner R, Kim J, Davies P, Wong STC. Driver Network as a Biomarker: Systematic integration and network modeling of multi-omics data to derive driver signaling pathways for drug combination prediction. Bioinformatics. 2019 Feb 15. pii: btz109. doi: 10.1093/bioinformatics/btz109. [Epub ahead of print] PubMed PMID: 30768150.

Chowdhury P, Powell RT, Stephan C, Uray IP, Talley T, Karki M, Tripathi DN, Park YS, Mancini MA, Davies P, Dere R. Bexarotene - a novel modulator of AURKA and the primary cilium in VHL-deficient cells. J Cell Sci. 2018 Dec 14;131(24). pii: jcs219923. doi: 10.1242/jcs.219923. PubMed PMID: 30518623; PubMed Central PMCID: PMC6307881.

Kalu NN, Mazumdar T, Peng S, Tong P, Shen L, Wang J, Banerjee U, Myers JN, Pickering CR, Brunell D, Stephan CC, Johnson FM. Comprehensive pharmacogenomic profiling of human papillomavirus-positive and -negative squamous cell carcinoma identifies sensitivity to aurora kinase inhibition in KMT2D mutants. Cancer Lett. 2018 Sep 1;431:64-72. doi: 10.1016/j.canlet.2018.05.029. Epub 2018 May 25. PubMed PMID: 29807113.

McGrail DJ, Lin CC, Dai H, Mo W, Li Y, Stephan C, Davies P, Lu Z, Mills GB, Lee JS, Lin SY. Defective Replication Stress Response Is Inherently Linked to the Cancer Stem Cell Phenotype. Cell Rep. 2018 May 15;23(7):2095-2106. doi: 10.1016/j.celrep.2018.04.068. PubMed PMID: 29768207.

Dong J, Park SY, Nguyen N, Ezhilarasan R, Martinez-Ledesma E, Wu S, Henry V, Piao Y, Tiao N, Brunell D, Stephan C, Verhaak R, Sulman E, Balasubramaniyan V, de Groot JF. The polo-like kinase 1 inhibitor volasertib synergistically increases radiation efficacy in glioma stem cells. Oncotarget. 2018 Jan 8;9(12):10497-10509. doi: 10.18632/oncotarget.24041. eCollection 2018 Feb 13. PubMed PMID: 29535822; PubMed Central PMCID: PMC5828226.

Kogiso M, Qi L, Lindsay H, Huang Y, Zhao X, Liu Z, Braun FK, Du Y, Zhang H, Bae G, Zhao S, Injac SG, Sobieski M, Brunell D, Mehta V, Tran D, Murray J, Baxter PA, Yuan XJ, Su JM, Adesina A, Perlaky L, Chintagumpala M, Parsons DW, Lau CC, Stephan CC, Lu X, Li XN. Xenotransplantation of pediatric low grade gliomas confirms the enrichment of BRAF V600E mutation and preservation of CDKN2A deletion in a novel orthotopic xenograft mouse model of progressive pleomorphic xanthoastrocytoma.Oncotarget. 2017 Sep 8;8(50):87455-87471. doi: 10.18632/oncotarget.20713. eCollection 2017 Oct 20. PubMed PMID: 29152094; PubMed Central PMCID: PMC5675646.

Kumar S, Peng X, Daley J, Yang L, Shen J, Nguyen N, Bae G, Niu H, Peng Y, Hsieh HJ, Wang L, Rao C, Stephan CC, Sung P, Ira G, Peng G. Inhibition of DNA2 nuclease as a therapeutic strategy targeting replication stress in cancer cells. Oncogenesis. 2017 Apr 17;6(4):e319. doi: 10.1038/oncsis.2017.15. PubMed PMID: 28414320; PubMed Central PMCID: PMC5520492.

Szafran AT, Stephan C, Bolt M, Mancini MG, Marcelli M, Mancini MA. High-Content Screening Identifies Src Family Kinases as Potential Regulators of AR-V7 Expression and Androgen-Independent Cell Growth. Prostate. 2017 Jan;77(1):82-93. doi: 10.1002/pros.23251. Epub 2016 Oct 4. PubMed PMID: 27699828; PubMed Central PMCID: PMC5956900.

Guan F, Bronk L, Titt U, Lin SH, Mirkovic D, Kerr MD, Zhu XR, Dinh J, Sobieski M, Stephan C, Peeler CR, Taleei R, Mohan R, Grosshans DR. Spatial mapping of the biologic effectiveness of scanned particle beams: towards biologically optimized particle therapy. Sci Rep. 2015 May 18;5:9850. doi: 10.1038/srep09850. PubMed PMID: 25984967; PubMed Central PMCID: PMC4650781.

Cohen T, Widdows D, Stephan C, Zinner R, Kim J, Rindflesch T, Davies P. Predicting high-throughput screening results with scalable literature-based discovery methods. CPT Pharmacometrics Syst Pharmacol. 2014 Oct 8;3:e140. doi: 10.1038/psp.2014.37. PubMed PMID: 25295575; PubMed Central PMCID: PMC4474167.

Lin SH, Zhang J, Giri U, Stephan C, Sobieski M, Zhong L, Mason KA, Molkentine J, Thames HD, Yoo SS, Heymach JV. A high content clonogenic survival drug screen identifies mek inhibitors as potent radiation sensitizers for KRAS mutant non-small-cell lung cancer. J Thorac Oncol. 2014 Jul;9(7):965-73. doi: 10.1097/JTO.0000000000000199. PubMed PMID: 24922006; PubMed Central PMCID: PMC4110054.

Zhang N, Scorsone K, Ge G, Kaffes CC, Dobrolecki LE, Mukherjee M, Lewis MT, Berg S, Stephan CC, Pati D. Identification and Characterization of Separase Inhibitors (Sepins) for Cancer Therapy. J Biomol Screen. 2014 Jul;19(6):878-89. doi: 10.1177/1087057114520972. Epub 2014 Feb 13. PubMed PMID: 24525869.

Ding Z, German P, Bai S, Feng Z, Gao M, Si W, Sobieski MM, Stephan CC, Mills GB, Jonasch E. Agents that stabilize mutated von Hippel-Lindau (VHL) protein: results of a high-throughput screen to identify compounds that modulate VHL proteostasis. J Biomol Screen. 2012 Jun;17(5):572-80. doi: 10.1177/1087057112436557. Epub 2012 Feb 21. PubMed PMID: 22357874; PubMed Central PMCID: PMC3895461.

Phatak SS, Stephan CC, Cavasotto CN. High-throughput and in silico screenings in drug discovery. Expert Opin Drug Discov. 2009 Sep;4(9):947-59. doi: 10.1517/17460440903190961. PubMed PMID: 23480542.

De Vriese AS, Tilton RG, Stephan CC, Lameire NH. Vascular endothelial growth factor is essential for hyperglycemia-induced structural and functional alterations of the peritoneal membrane. J Am Soc Nephrol. 2001 Aug;12(8):1734-41. PubMed PMID: 11461947.

de Vriese AS, Tilton RG, Elger M, Stephan CC, Kriz W, Lameire NH. Antibodies against vascular endothelial growth factor improve early renal dysfunction in experimental diabetes. J Am Soc Nephrol. 2001 May;12(5):993-1000. PubMed PMID: 11316858.

Tilton RG, Chang KC, LeJeune WS, Stephan CC, Brock TA, Williamson JR. Role for nitric oxide in the hyperpermeability and hemodynamic changes induced by intravenous VEGF. Invest Ophthalmol Vis Sci. 1999 Mar;40(3):689-96. PubMed PMID: 10067972.

Rege AA, Bjercke RJ, Erichsen D, Owens R, Stephan CC, Brock TA. Development of novel monoclonal antibodies for the analysis of functional sites in FGF-2. Growth Factors. 1999;16(3):161-9. PubMed PMID: 10372957.

Stephan CC, Chang KC, LeJeune W, Erichsen D, Bjercke RJ, Rege A, Biediger RJ, Kogan TP, Brock TA, Williamson JR, Tilton RG. Role for heparin-binding growth factors in glucose-induced vascular dysfunction. Diabetes. 1998 Nov;47(11):1771-8. PubMed PMID: 9792547.

Tilton RG, Kawamura T, Chang KC, Ido Y, Bjercke RJ, Stephan CC, Brock TA, Williamson JR. Vascular dysfunction induced by elevated glucose levels in rats is mediated by vascular endothelial growth factor. J Clin Invest. 1997 May 1;99(9):2192-202. PubMed PMID: 9151791; PubMed Central PMCID: PMC508049.

Cunningham SA, Stephan CC, Arrate MP, Ayer KG, Brock TA. Identification of the extracellular domains of Flt-1 that mediate ligand interactions. Biochem Biophys Res Commun. 1997 Feb 24;231(3):596-9. PubMed PMID: 9070853.

Stephan CC, Brock TA. Vascular endothelial growth factor, a multifunctional polypeptide. P R Health Sci J. 1996 Sep;15(3):169-78. Review. PubMed PMID: 8994281.

Stephan CC, Sastry BV. Characterization of the subtype of muscarinic receptor coupled to the stimulation of phosphoinositide hydrolysis in 132-1N1 human astrocytoma cells. Cell Mol Biol (Noisy-le-grand). 1992 Nov;38(7):701-12. PubMed PMID: 1282058.

Stephan CC, Sastry BV. Subtype of muscarinic receptor coupled to the attenuation of hormone-stimulated cAMP accumulation in NG108-15 neuroblastoma x glioma hybrid cells. Cell Mol Biol (Noisy-le-grand). 1992 Aug-Sep;38(5-6):601-12. PubMed PMID: 1282846.

Elton TS, Stephan CC, Taylor GR, Kimball MG, Martin MM, Durand JN, Oparil S. Isolation of two distinct type I angiotensin II receptor genes. Biochem Biophys Res Commun. 1992 Apr 30;184(2):1067-73. PubMed PMID: 1575725.