Education and Training
2003 Bachelor of Medicine (M.D. Equivalent),
Zhejiang University, School of Medicine
2008 Ph.D. & M.S., Biochemistry, Georgia State University,
2009 Research Fellow, Immune Disease Institute,
Harvard Medical School, Boston, MA
Program in Cellular and Molecular Medicine at
Children’s Hospital Boston
2010 Research Fellow, La Jolla Institute for Allergy and
Immunology, La Jolla, CA
Imbalanced DNA methylation/demethylation results in abnormal gene expression and increased genome instability, which is frequently observed in human cancers. Dr. Huang ’s research interests are directed towards understanding how DNA methylation/demethylation balance is maintained in mammals and how aberrant DNA methylation and its oxidation products contribute to human cancer. Dr. Huang’s ongoing research includes using high-throughput sequencing to study the DNA modifications in human cancer, elucidating the role of DNA modification enzymes in mouse models, and exploring novel anti-cancer or preventive strategies by targeting key epigenetic pathways.
Dr. Huang has significantly contributed to the functional characterization of the ten eleven translocation (TET) enzymes in hematological malignancies. Dr. Huang also pioneered the use of innovative tools to probe the “sixth DNA base”, 5-hydroxymethylcytosine (5hmC), in the human genome and was among the first to profile the hydroxylmethylome in mouse embryonic stem cells. Her recent studies related to TET enzyme and 5hmC resulted in more than 15 peer-reviewed publications in top scientific journals and over 1,700 citations. She was recruited to the Texas A&M Health Science Center Institute of Biosciences & Technology as a CPRIT scholar in 2014.
He L, Zhang Q, Zhou Y, Huang, Y. Optogenetic approaches to control calcium entry in non-excitable cells. Calcium Entry Channels in Non-Excitable Cells. Methods in Signal Transduction Series. CRC Press, 2016.
Basanta-Sanchez M, Wang R, Liu Z, Ye X, Agris P, Li M, Shi X, Agris P, Zhou Y, Huang Y and Sheng J. TET1-mediated oxidation of 5-formylcytosine (5fC) to 5-carboxycytosine (5caC) in RNA. ChemBioChem, 2017 Jan 3;18(1):72-76.
Zhang X, Su J, Jeong M, Ko M, Huang Y, Park H, Guzman A, Lei Y, Huang Y, Rao A, Li W, Goodell M. DNMT3A and TET2 compete and cooperate to repress lineage-specific factors in hematopoietic stem cells. Nature Genetics, 2016 Sep;48(9):1014-23.
Huang Y, Chavez L, Xing C, Wang X, Pastor WA, Kang J, Zepeda-Martínez JA, Pape UJ, Jacobsen SE, Peters B, Rao A. Distinct roles of the methylcytosine oxidases Tet1 and Tet2 in mouse embryonic stem cells. Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1361-6.
Chavez L, Huang Y, Luong K, Agarwal S, Iyer LM, Pastor WA, Hench VK, Frazier-Bowers SA, Korol E, Liu S, Tahiliani M, Wang Y, Clark TA, Korlach J, Pukkila PJ, Aravind L, Rao A. Simultaneous sequencing of oxidized methylcytosines produced by TET/JBP proteins in Coprinopsis cinerea. Proc Natl Acad Sci U S A. 2014 Dec 2;111(48):E5149-58.
Huang Y, Rao A. Connections between TET proteins and aberrant DNA modification in cancer. Trends in Genetics. 2014 Oct;30(10):464-74.
Huang Y, Pastor WA, Zepeda-Martinez A. J, Anjana Rao. The anti-CMS technique for genome-wide mapping of 5-hydroxymethylcytosine. Nature Protoc. 2012 Oct;7(10):1897-908.
Pastor WA, Pape UJ, Huang Y, Henderson HR, Lister R, Ko M, McLoughlin EM, Brudno Y, Mahapatra S, Kapranov P, Tahiliani M, Daley GQ, Liu XS, Ecker JR, Milos PM, Agarwal S, Rao A. Genome-wide mapping of 5-hydroxymethylcytosine in embryonic stem cells. Nature. 2011 May 19;473(7347):394-7.
Ko M, Huang Y, Jankowska AM, Pape UJ, Tahiliani M, Bandukwala HS, An J, Lamperti ED, Koh KP, Ganetzky R, Liu XS, Aravind L, Agarwal S, Maciejewski JP, Rao A. Impaired hydroxylation of 5-methylcytosine in myeloid cancers with mutant TET2. Nature. 2010 Dec 9;468(7325):839-43.
Huang Y, Pastor WA, Shen Y, Tahiliani M, Liu DR, Rao A. The behavior of 5-hydroxymethylcytosine in bisulfite sequencing. PLoS One. 2010 Jan 26;5(1):e8888.