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Protein expression and  purification

Expression usually will be done with the optimized conditions in Terrific Broth media.

Purification will be done by using affinity chromatography, ion exchange and gel-filtration (SEC) on AKTA Pure system. Tag can be removed (in order to get tag-free protein) on request with an additional cost of $400-1,000 (depends on the expression scale; besides, the final recovery varies with the characteristics of the protein).

 

2-L scale: $1,200

4-L scale: $1,600

6-L scale: $1,900

12-L scale: $2,600