The list below summarizes our comprehensive service offerings at the core facility

  • Custom cloning service (Genomic DNA clone; cDNA clone; genetic engineering)
  • Site-directed mutagenesis (Single mutation and multiple mutations in a single construct; library of random mutants)
  • Expression optimization tests
  • Pilot scale protein expression and purification
  • Large-scale protein production
  • Co-expression and purification of protein complexes
  • Protein refolding from inclusion bodies
  • Tag removal for purified recombinant proteins
  • SDS-PAGE & Western Blot assays for expressed recombinant proteins
  • Size-exclusion chromatography (GE Healthcare’s prepacked SEC columns from analytic scale Superdex increase to production grade Superdex) on ÄKTA Pure system
  • Affinity chromatography (GE Healthcare’s prepacked affinity columns, i.e. HisTrap HP, StrepTrap HP, GSTrap HP, MBPTrap HP) on ÄKTA Pure system
  • Endotoxin removal for purified recombinant proteins
  • Multiple-step protein purification on ÄKTA Pure system
  • Protein activity assay
  • Protein crystallization screening, optimization, and scale-up; customers also can rent time on Mosquito LCP and Dragonfly screen optimizer with our supervision and assistance.
  • Spectroscopy based biophysics assays (CD, ITC and SPR); customers also can rent time on MicroCal PEAQ-ITC and Biacore T200 with our supervision and assistance.