The IBT Flow Cytometry and Cell Sorting Facility (FCCSF) provides diverse, technically sophisticated flow cytometry and cell sorting services, as well as the scientific expertise necessary to integrate this technology into research projects (Dr. Moczygemba).

 Flow Cytometry


Major Equipment

BD Biosciences FACSFusion Cell sorter:

  • located in room 419 of the IBT Alkek building
  • our sorter uses electrostatic droplet-based, high speed technology for isolating any combination of fluorescently labeled cell markers
  • equipped with 4 lasers (405 nm, 488 nm, 561 nm, and 640 nm) and 15 filters for experiments requiring up to 15 multicolor analysis
  • we have a variety of nozzle sizes/pressure configurations for sorting all types of cells including fragile ones
  • can sort up to 50,000 events/second in temperature-controlled sample and collection chamber  
  • instrument is certified to perform BSL2 level sorting of single cells into 96 well plates, as well as bulk sorting into two or four tubes
  • users are encouraged to contact Dr. Moczygemba or Ms. Isgandarova during the planning stages of their cell sorting experiment


BD Biosciences LSRII analytical flow cytometer:

  • located in room 719 IBT Alkek building
  • equipped with 4 lasers (405 nm, 488 nm, 561 nm, and 640 nm) and 13 filters suitable for experiments requiring up to 13 color analysis plus forward and side scatter data acquisition
  • for complex flow cytometry assays or if simply developing a new assay, users are encouraged to contact Dr. Moczygemba or Ms. Isgandarova during the planning stages of their experiment
  • users have access to FlowJo data analysis software to customize their data analysis

 

Flow cytometry analyses commonly performed:

  • cell sorting from cell lines, primary cells and disaggregated tissues
  • immunophenotyping, cellular cytotoxicity assays, apoptosis detection, kinetic studies, receptor endocytosis, cell cycle analysis, bacterial analysis, and others as needed.

 

Future instruments:

Intellicyte iQue Screener Plus

  • will provide access to high throughput flow cytometry analysis for drug discovery research
  • will support plate-based sampling systems (96- or 384-well plates) that can be integrated with plate-handling robotics and automated cell culture and liquid-handling peripherals
  • when fully automated, flow cytometry workstations have a maximal throughput capacity of up to 72 separate 384-well plates per day, this is a 50-100 fold improvement over the throughput of conventional flow cytometers


Core Leadership

Image of Margie Moczygemba, PhD
Margie Moczygemba, PhD
Assistant Professor
Core Director
Center for Infectious and Inflammatory Diseases
713.677.8114
mmoczygemba@tamhsc.edu