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Flow Cytometry Core Laboratory - Service Request Form

Check FACS calendar. Fill out form below, confirm, then submit. Email confirmation will be sent and the request status can be viewed online. Service request approval is dependent upon the submission of the Biosafety Form.

If you experience any problems with this form please contact Kay Kendall at kkendall@ibt.tamhsc.edu .

Note: *All fields are required.
Flow Cytometry Core Laboratory
Requester Name: Request Date: //
Phone: Start Time:
Email: End Time:
Principal Investigator: Center:
Please briefly describe your experiment:
Analysis:
Immunophenotyping Apoptosis
Cell cycle Other:  
Sorting:
Sterile Non-sterile
Cell Type:
Established         Primary         Embryonic
(if possible please provide the source, ie. company, academic institution)
Source:
Species:
Human Non-Human mammalian Other
Required description for Non-Human mammalian only:
Tissue origin:
Organ(s): Bone marrow
Other: Peripheral blood
Estimated cell size:
< 10um 10-20um > 20um
Total number cells/tube or sample Volume/tube or sample (ml)
Note: Optimum cell concentration at sort rate of 1000-2000 events is 1-2x106 cells/ml.
Suspension buffer: PBS      HBSS      Other:
Note: In order to prevent clogging of the nozzle to avoid delays, suspension buffer must not contain serum (very sticky) and calcium (Ca2+ reacts with the phosphate in the 1X PBS sheath fluid forming precipitates), and samples must be filtered through a nylon mesh or cell strainer to remove aggregates. HBSS is ideal for maintaining the pH for continuous sorts lasting longer than 1hr.
Detection: (Fluorochromes - dyes, stains, and fluorescent proteins)
Requires at least one detector.  Click here to view a list detectors
FL1: (filter/range) 510/21, 530/30 (wavelength in nm)
FL2: 550/30, 575/26, 585/42
FL3: 630/22, 660/20, 675/20
FL4: 695/40, 712/21 Or FL4 UV: 424/44
For Flow Cytometer Lab Use Only

Service Reference No. ____________________ Date: _______________________
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